antiannexin a1 antibody  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: GPCRs overexpression and impaired fMLP-induced functions in neutrophils from chronic kidney disease patients

doi: 10.3389/fimmu.2024.1387566

Figure Lengend Snippet: CKD neutrophils overexpress intracellular AnxA1. Increased FPR2 expression in CKD neutrophils (A) . AnxA1 expression in neutrophils at intracellular (B) and membrane compartments (C) . AnxA1 secretion from neutrophils (D) and apoptotic neutrophils (E) cultured for 18 hours under serum starvation conditions. (Control n= 3–11 and CKD n= 3–36) **p<0.01; ***p<0.001 CKD vs Control. ns, Not significant.

Article Snippet: Patients’ samples were incubated with anti-AnxA1 goat antibody (1:100) (AF3770; R&D SYSTEM) and anti-myeloperoxidase rabbit antibody (1:50; Biodesign International, Inc., Maine, USA) overnight at 4°C, followed by incubation with anti-goat Alexa Fluor 467 and anti-rabbit Alexa Fluor 488 secondary antibodies (1:200; Invitrogen).

Techniques: Expressing, Membrane, Cell Culture, Control

Journal: Frontiers in Immunology

Article Title: GPCRs overexpression and impaired fMLP-induced functions in neutrophils from chronic kidney disease patients

doi: 10.3389/fimmu.2024.1387566

Figure Lengend Snippet: Infiltrate neutrophils in CKD kidney biopsies are AnxA + . H&E (A–E) and PAS staining (F–J) . Myeloperoxidase (MPO) and AnxA1 labeling (K–O) . Five CKD biopsies were selected and stained for morphological, fibrosis visualization, and immunofluorescence. Figure 5D – Insert: * immune cells infiltrated; MPO and AnxA1 labeling. The image is representative of at least three fields analyzed on each biopsy.

Article Snippet: Patients’ samples were incubated with anti-AnxA1 goat antibody (1:100) (AF3770; R&D SYSTEM) and anti-myeloperoxidase rabbit antibody (1:50; Biodesign International, Inc., Maine, USA) overnight at 4°C, followed by incubation with anti-goat Alexa Fluor 467 and anti-rabbit Alexa Fluor 488 secondary antibodies (1:200; Invitrogen).

Techniques: Staining, Labeling, Immunofluorescence

Journal: bioRxiv

Article Title: Exosomes are vehicles for the stress-regulated secretion of histones

doi: 10.1101/2024.04.08.588575

Figure Lengend Snippet: (A) WBs showing the effects of siRNA mediated knock-down of Alix , Tsg101 , and Smpd2 in OLN-93 cells. H3 and EV markers (Alix, Tsg101 as well as the plasma membrane/microvesicle marker Annexin A1) were quantified in total protein recovered from the media by StrataClean pulldown. The experiment was repeated 4 times, and a representative blot is shown. Band intensities were quantified, and the column chart below shows the ratio of H3 (treated/control) from four independent experiments. (B) Pharmacological treatment of OLN-93 cells, analysed as in (A). Control is without treatment. (C) Immuno-TEM micrographs showing anti-H1 labelling associated with ILVs in OLN-93 cell sections, following treatment with compounds that significantly increased (3-MA or bafilomycin) or decreased (sodium butyrate) H3 secretion into the media. Right, scatter plot showing quantification of histone positive ILVs under stress conditions. Two-way ANOVA and Bonferroni multiple comparison test was performed: ***, p < 0.0001, **, p < 0.001, *, < 0.05.

Article Snippet: Annexin A1 (AF3770, R&D Systems).

Techniques: Knockdown, Clinical Proteomics, Membrane, Marker, Control, Comparison